Biological female and transgender female. The difference between the biological female and the transgender female is that the biological female is born with female anatomy. After recognizing and transitioning in some way to reflect her femininity, the transgender female differs from a biological female only in her origins (illustrated here by the color blue).
A Biological Female (to the left, in pink), is a woman who is born with the corresponding female anatomy, has feminine behavioral patterns, and identifies as female on a personal level. A Transgender Female (to the right, in blue), is a woman who is born without the corresponding female anatomy (born with male anatomy), but still has feminine behavioral patterns, and identifies as female on a personal level.
Read more about Physical Gender v. Gender Identity.